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Basic Science - Human Intervertebral Disc Cells Are Genetically Modifiable by Adenovirus-Mediated Gene Transfer - Human intervertebral disc cells were cultured in monolayer and treated with adenovirus containing marker genes to determine the susceptibility of the cells to adenovirus, mediated gene transfer. Adenovirus efficiently transferred lacZ and luciferase marker genes to cells from degenerated discs as well as to cells from nondegenerated discs. In vitro transducibility was relatively insensitive to disc degeneration grade./
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