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에탄올이 뇌 신경세포주 B103의 Protein Kinase C 활성에 미치는 영향

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상세정보
자료유형	학위논문
개인저자	조효정. 정영진
서명/저자사항	에탄올이 뇌 신경세포주 B103의 Protein Kinase C 활성에 미치는 영향/趙孝貞.
발행사항	대전: 충남대학교 대학원, 2000.
형태사항	iv,43 p.: 삽도; 26 cm.
딸림자료
대등서명	Effect of ethanol on the PKC levels in B103 cells from the rat central nervous system

학위논문주기	학위논문(석사) --충남대학교 대학원:식품영양학과 영양학전공,2000
일반주기	지도교수: 丁英鎭
서지주기	참고문헌 : p.34-40.
언어	한국어

소장정보

소장정보

분관대출 서가에 책이 없을 때... 도서 Pick-Up서비스 찾아가는대출서비스 인쇄

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No.	등록번호	청구기호	소장처	도서상태	반납예정일	예약	서비스
1	DM131578	T 1G2 000-1	중앙도서관/3층 학위논문실/	대출불가(별치)
2	DM131579	T 1G2 000-1 c.2	중앙도서관/3층 학위논문실/	대출불가(별치)

초록

초록

초록 일부

초록 전체

Alcohol is the most used psychiotropic drug. It is well known that long-term heavy alcohol(ethanol) intake causes alcoholic dementia, cerebellar degeneracy or Wernicke-korsakoff Syndrome and aggravates the conditions of many other neuro-psychotic disorders.  Despite the serious problems associated with ethanol, there is little research on the

* A thesis submitted to the committee of Graduate School, Chungnam National University in partial fulfillment of the requirements for the degree of Master of Home Economics conferred in February 2000.
reaction mechanism of ethanol on the central nervous system.  Recently it is indicated that PKC plays an important role in the action of ethanol and in the neuroadaptational mechanisms under chronic ethanol exposure.  Accordingly this study was carried out to investigate the effect of ethanol in the range of not showing any cytotoxicity on the PKC isoform levels in B103 cells from the rat central nervous system using western blot analysis and PKC isoform-specific antibodies.  The changes of PKC α, β, γ, ε and ζ level in the range of ethanol concentration 50, 100 and 200mM were examined at the exposure time 1, 2, 8, 18 and 24hrs and in both fractions (cytosolic and membrane fraction).
  PKC α turned out to be decreased in the cytosolic fraction at 1, 2 and 8hrs ethanol exposure times in all the ethanol concentrations but increased in both cytosolic and membrane fractions at 24hr ethanol exposure time in 200mM ethanol concentration.  PKC β was not detected, however PKC γ was significantly decreased in both cytosolic and membrane fractions at 24hr ethanol exposure time in all the ethanol concentrations.  PKC ε was decreased at all the ethanol exposure times in all the ethanol concentrations in the cytosolic fraction and at 24hr ethanol exposure time in any ethanol concentrations, the trend of decreased was observed in the membrane fraction.  Lastly the change of  PKC ζ level did not show any consistency.
  These results altogether indicate that in the range of ethanol concentration not show cytotoxicity, ethanol may modulate signal transduction and neurotransmitter release in the central nervous system, through the change of PKC isoform level, and the action of these isoforms may be different each other in the cell by showing the different pattern of change according to the PKC isoform type.  In addition, these results imply that the behavioral abnormality of inhibition or excitation of central nervous system caused by ethanol may appeared through the change of signal transduction system PKC involved.

Ⅰ.서 론     1

Ⅱ. 실험재료 및 방법    7

1. 실험재료 및 사용기기    7
   1.1. 시약 및 세포 배양 용기    7
   1.2. 사용기기    8
2. 실험 방법    9
   2.1. 세포 배양    9
   2.2. 세포 증식 실험    9
   2.3. Ethanol 처리 및 전기영동시료 제조    10
     2.3.1  Ethanol 처리    ...